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Assistant Professor Patrick Cirino | Selected Publications


  1. Fazelinia H, Cirino PC, and Maranas CD. OptGraft: A computational procedure for transferring a binding site onto an existing protein scaffold (Submitted).
  2. Frei CS and Cirino PC. Combinatorial Enzyme Engineering. In: Protein Engineering and Design. Cochran JR and Park S, Eds. Taylor and Francis: 2009.
  3. Akinterinwa O, Khankal R and Cirino PC. Metabolic Engineering for Bioproduction of Sugar Alcohols, Current Opinion in Biotechnology (Invited Review, Submitted).
  4. Akinterinwa O and Cirino PC. Heterologous expression of D-xylulokinase from Pichia stipitis enables high levels of xylitol production by engineered Escherichia coli growing on xylose. 2008, (Submitted)
  5. Chin JW, Khankal R, Monroe, CA, Maranas CD and Cirino PC. Analysis of NADPH supply during xylitol production by engineered Escherichia coli. Biotechnol. Bioeng. (Accepted)
  6. Khankal R*, Luziatelli F* (*equal contributions), Chin JW, Frei CS and Cirino PC. Comparison between Escherichia coli K-12 strains W3110 and MG1655 and wild-type E. coli B as platforms for xylitol production. Biotechnol. Lett. 2008, (Accepted)
  7. Tang SY, Fazelinia H, and Cirino PC. AraC regulatory protein mutants with altered effector specificity. J. Am. Chem. Soc. 2008, April 16; 130(15): 5267-71
  8. Weiss LE, Badalamenti JP, Weaver JL, Tascone AR, Weiss PS, Richard TL and Cirino PC, Engineering motility as a phenotypic response to LuxI/R-dependent quorum sensing in Escherichia coli. Biotechnol. Bioeng. 2008, 100(6): 1251-5
  9. Cirino PC and Sun L. Advancing Biocatalysis through Enzyme, Cellular, and Platform Engineering. Biotechnol. Progr. 2008, May-June; 24(3): 515-9
  10. Khankal R, Chin JW, Cirino PC. Role of xylose transporters in Escherichia coli engineered for xylitol production. J. Biotechnology. 2008, April 30, 134(3-4): 246-52
  11. Badalamenti JP, Weiss LE, Buckno CJ, Richard TL, Weiss PS and Cirino PC. Synthetic sports: a bacterial relay race. IET Synth. Biol., 2007, 1:61.
  12. Akinterinwa O and Cirino PC. Catabolism and Metabolic Fueling Processes In: Handbook for Metabolic Pathway Engineering. Smolke CD, Ed. CRC Press: 2008.
  13. Fazelinia H, Cirino PC, and CD Maranas. Extending IPRO in protein library design for ligand specificity. Biophys. J. 2007, 92(6):2120.
  14. Cirino PC, Chin JW and Ingram LO. Engineering Escherichia coli for Xylitol Production from Glucose-Xylose Mixtures. Biotechnol. Bioeng. 2006, 95: 1167.
  15. Cirino PC and Arnold FH. A self-sufficient peroxide-driven hydroxylation biocatalyst. Angew. Chem.-Int. Edit. 2003, 42: 3299.
  16. Salazar O*, Cirino PC* (*equal contributions) and Arnold FH. Thermostabilization of a Cytochrome P450 Peroxygenase. ChemBioChem. 2003, 4: 891.
  17. Cirino PC, Tang Y, Takahashi K, Tirrell DA and Arnold FH. Global Incorporation of Norleucine in Place of Methionine in Cytochrome P450 BM-3 Heme Domain Increases Peroxygenase Activity. Biotechnol Bioeng. 2003, 83: 729.
  18. Cirino PC, Mayer KM, and Umeno D. Generating mutant libraries using error-prone PCR. In: Directed Evolution Library Creation: Methods and Protocols. Arnold and Georgiou, Eds, Humana Press; 2003.
  19. Cirino PC and Georgescu R. Screening for thermostability. In: Directed Evolution Library Creation: Methods and Protocols. Arnold and Georgiou, Eds, Humana Press; 2003.
  20. Cirino PC and Arnold FH. Regioselectivity and activity of cytochrome P450 BM-3 and mutant F87A in reactions driven by hydrogen peroxide. Adv. Synth. Catal. 2002, 344: 932.
  21. Cirino PC and Arnold FH. Protein engineering of oxygenases for biocatalysis. Curr. Opin. Chem. Bio. 2002, 6: 130.
  22. Cirino PC and Arnold FH. Exploring the diversity of heme enzymes through directed evolution. In: Directed Molecular Evolution of Proteins. Brakmann and Johnsson, Eds, Wiley-VCH, Weinheim, 2002.
  23. Schwaneberg U, Otey C, Cirino PC, Farinas E, Arnold FH. Cost-effective whole-cell assay for laboratory evolution of hydroxylases in Escherichia coli. J. Biomol. Screen. 2001, 6: 111.
  24. Morawski B, Lin ZL, Cirino P, Joo H, Bandara G, Arnold FH. Functional expression of horseradish peroxidase in Saccharomyces cerevisiae and Pichia pastoris. Protein Eng. 2000, 13: 377.

Updated on 07/21/08,   Top